sypro ruby protein blot stain (Thermo Fisher)
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Thermo Fisher
sypro ruby protein blot stain
Sypro Ruby Protein Blot Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sypro+ruby+protein+blot+stain/sypro+orange+protein+gel+stain/pmc12274804-402-21-26
Average 90 stars, based on 1 article reviews
Sypro Ruby Protein Blot Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sypro+ruby+protein+blot+stain/sypro+orange+protein+gel+stain/pmc12274804-402-21-26
Average 90 stars, based on 1 article reviews
sypro ruby protein blot stain - by Bioz Stars,
2026-09
90/100 stars
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Staining:Article Title: MYC is a regulator of androgen receptor inhibition-induced metabolic requirements in prostate cancer Article Snippet: Samples were run on NuPAGE 4%−12% Bis-Tris Gels (Thermo Fisher Scientific, NP0335) and protein was transferred to PVDF transfer membranes (Thermo Fisher Scientific, IPV00010). .. Total protein was visualized using the Article Title: OGDHL regulates nucleotide metabolism, tumor growth, and neuroendocrine marker expression in prostate cancer Article Snippet: Samples were run on NuPAGE 4-12% Bis-Tris Gels (ThermoFisher Scientific: NP0335, NP0322) at 200V for 40-50 minutes, then transferred to ImmobilonTM-P PVDF Membranes (Millipore Sigma: IPVH 00010) at 30V for 60 minutes. .. Total protein was visualized using the Article Title: Human iPSC-based disease modeling studies identify a common mechanistic defect and potential therapies for AMD and related macular dystrophies. Article Snippet: Article Human iPSC-based disease modeling studies identify a common mechanistic defect and potential therapies for AMD and related macular dystrophies Article Title: Molecular Determinants of Protein Pathogenicity at the Single-Aggregate Level. Article Snippet: Dot Blot Assay: It was loaded 750 ng of Aβ42 peptide onto a 0.2 μm nitrocellulose membrane of the Bio-Dot Apparatus (Bio-Rad Cat. No. 1 706 545). .. Then the membrane washed twice with TBS containing 2% formaldehyde and then incubated with the same solution for 30 min. Then the membrane was rinsed with deionized water thrice. fixed with 7% MeCOOH and 10% MeOH for 15 min and stained with Article Title: Determining the ERK-regulated phosphoproteome driving KRAS-mutant cancer Article Snippet: The commercially available GenPoint kit (Agilent Technologies) based on tyramine avidin/biotin signal amplification coupled with the fluorescent IRDye 680 Streptavidin (Li-Cor Biosciences) was used for signal detection. .. The total protein amount for each sample was assessed by staining one in every 15 slides with Article Title: Novel tools to quantify total, phospho-Ser129 and aggregated alpha-synuclein in the mouse brain. Article Snippet: For quantitation of synaptophysin in mouse brain extracts, 7 μg of total protein was incubated in loading buffer as described above and loaded into AnykDMini-PROTEANTGX Precast Protein Gels (15 wells, 15 μL; Bio-Rad). .. Electrophoresis and protein transfer were performed as above, without membrane fixation, before membranes were dried overnight 2 h at room temperature and proteins stained with Article Title: Estrogen-mediated corneal collagen degradation in keratoconus Article Snippet: Samples were diluted in 4x Laemmli SDS sample reducing buffer (Thermo Fisher Scientific) and heated at 90 o C for 10 min. Denatured protein samples were then resolved on 4–20% Tris-HCl gradient gels (Bio-Rad, California, USA) and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore Sigma). .. Prior to analyzing levels of specific proteins using anti bodies, total protein levels on the PVDF membranes post-transfer were evaluated using Article Title: Integrative Multi-Omics Approach in Vascular Ehlers-Danlos Syndrome: Further Insights into the Disease Mechanisms by Proteomic Analysis of Patient Dermal Fibroblasts. Article Snippet: The membranes were blocked for 3 h at RT in 5% non-fat dry milk/TBS-T and immunoreacted overnight at +4 ◦C with 1:1000 rabbit anti-human COL5A1 antibody (US Biological Life Science, Salem, MA, USA, #221804) diluted in 5% non-fat dry milk/TBS-T. After washing in TBS-T, membranes were incubated for 3 h at RT with HRP-conjugated anti-goat IgGs (Merck Life Science, #401515) and anti-rabbit IgGs (Merck Life Science, #A8275), respectively, diluted 1:1000 in 5% non-fat dry milk/TBS-T. Chemiluminescent signals were developed using the ECL method (Thermofisher Scientific, #34580). .. Band quantification was performed using the Image Pro Plus software version 6.2 (Media Cybernetics), and the integrated optic density values were normalized against the total amount of proteins transferred on the membrane in the same lane, stained by Fluorescence:Article Title: Human iPSC-based disease modeling studies identify a common mechanistic defect and potential therapies for AMD and related macular dystrophies. Article Snippet: Article Human iPSC-based disease modeling studies identify a common mechanistic defect and potential therapies for AMD and related macular dystrophies Membrane:Article Title: Molecular Determinants of Protein Pathogenicity at the Single-Aggregate Level. Article Snippet: Dot Blot Assay: It was loaded 750 ng of Aβ42 peptide onto a 0.2 μm nitrocellulose membrane of the Bio-Dot Apparatus (Bio-Rad Cat. No. 1 706 545). .. Then the membrane washed twice with TBS containing 2% formaldehyde and then incubated with the same solution for 30 min. Then the membrane was rinsed with deionized water thrice. fixed with 7% MeCOOH and 10% MeOH for 15 min and stained with Article Title: Novel tools to quantify total, phospho-Ser129 and aggregated alpha-synuclein in the mouse brain. Article Snippet: For quantitation of synaptophysin in mouse brain extracts, 7 μg of total protein was incubated in loading buffer as described above and loaded into AnykDMini-PROTEANTGX Precast Protein Gels (15 wells, 15 μL; Bio-Rad). .. Electrophoresis and protein transfer were performed as above, without membrane fixation, before membranes were dried overnight 2 h at room temperature and proteins stained with Article Title: Integrative Multi-Omics Approach in Vascular Ehlers-Danlos Syndrome: Further Insights into the Disease Mechanisms by Proteomic Analysis of Patient Dermal Fibroblasts. Article Snippet: The membranes were blocked for 3 h at RT in 5% non-fat dry milk/TBS-T and immunoreacted overnight at +4 ◦C with 1:1000 rabbit anti-human COL5A1 antibody (US Biological Life Science, Salem, MA, USA, #221804) diluted in 5% non-fat dry milk/TBS-T. After washing in TBS-T, membranes were incubated for 3 h at RT with HRP-conjugated anti-goat IgGs (Merck Life Science, #401515) and anti-rabbit IgGs (Merck Life Science, #A8275), respectively, diluted 1:1000 in 5% non-fat dry milk/TBS-T. Chemiluminescent signals were developed using the ECL method (Thermofisher Scientific, #34580). .. Band quantification was performed using the Image Pro Plus software version 6.2 (Media Cybernetics), and the integrated optic density values were normalized against the total amount of proteins transferred on the membrane in the same lane, stained by Incubation:Article Title: Molecular Determinants of Protein Pathogenicity at the Single-Aggregate Level. Article Snippet: Dot Blot Assay: It was loaded 750 ng of Aβ42 peptide onto a 0.2 μm nitrocellulose membrane of the Bio-Dot Apparatus (Bio-Rad Cat. No. 1 706 545). .. Then the membrane washed twice with TBS containing 2% formaldehyde and then incubated with the same solution for 30 min. Then the membrane was rinsed with deionized water thrice. fixed with 7% MeCOOH and 10% MeOH for 15 min and stained with Electrophoresis:Article Title: Novel tools to quantify total, phospho-Ser129 and aggregated alpha-synuclein in the mouse brain. Article Snippet: For quantitation of synaptophysin in mouse brain extracts, 7 μg of total protein was incubated in loading buffer as described above and loaded into AnykDMini-PROTEANTGX Precast Protein Gels (15 wells, 15 μL; Bio-Rad). .. Electrophoresis and protein transfer were performed as above, without membrane fixation, before membranes were dried overnight 2 h at room temperature and proteins stained with Software:Article Title: Integrative Multi-Omics Approach in Vascular Ehlers-Danlos Syndrome: Further Insights into the Disease Mechanisms by Proteomic Analysis of Patient Dermal Fibroblasts. Article Snippet: The membranes were blocked for 3 h at RT in 5% non-fat dry milk/TBS-T and immunoreacted overnight at +4 ◦C with 1:1000 rabbit anti-human COL5A1 antibody (US Biological Life Science, Salem, MA, USA, #221804) diluted in 5% non-fat dry milk/TBS-T. After washing in TBS-T, membranes were incubated for 3 h at RT with HRP-conjugated anti-goat IgGs (Merck Life Science, #401515) and anti-rabbit IgGs (Merck Life Science, #A8275), respectively, diluted 1:1000 in 5% non-fat dry milk/TBS-T. Chemiluminescent signals were developed using the ECL method (Thermofisher Scientific, #34580). .. Band quantification was performed using the Image Pro Plus software version 6.2 (Media Cybernetics), and the integrated optic density values were normalized against the total amount of proteins transferred on the membrane in the same lane, stained by |